Dlx3 and Dlx5 Inhibit Adipogenic Differentiation of Human Dental Pulp Stem Cells.
- Author:
Hye Lim LEE
1
;
Hyun NAM
;
Gene LEE
;
Jeong Hwa BAEK
Author Information
1. Department of Molecular Genetics, School of Dentistry and Dental Research Institute, Seoul National University, Seoul 110-749, Korea. baekjh@snu.ac.kr
- Publication Type:Original Article
- Keywords:
Dlx3;
Dlx5;
adipocyte differentiation;
human dental pulp stem cells
- MeSH:
Dental Pulp;
Durapatite;
Genes, Homeobox;
Humans;
Lipoprotein Lipase;
Mesenchymal Stromal Cells;
Osteoblasts;
PPAR gamma;
Proteins;
RNA, Messenger;
Stem Cells
- From:International Journal of Oral Biology
2012;37(1):31-36
- CountryRepublic of Korea
- Language:English
-
Abstract:
Dlx3 and Dlx5 are homeobox domain proteins and are well-known regulators of osteoblastic differentiation. Since possible reciprocal relationships between osteogenic and adipogenic differentiation in mesenchymal stem cells exist, we examined the regulatory role of Dlx3 and Dlx5 on adipogenic differentiation using human dental pulp stem cells. Over-expression of Dlx3 and Dlx5 stimulated osteogenic differentiation but inhibited adipogenic differentiation of human dental pulp stem cells. Dlx3 and Dlx5 suppressed the expression of adipogenic marker genes such as C/EBPalpha, PPARgamma, aP2 and lipoprotein lipase. Adipogenic stimuli suppressed the mRNA levels of Dlx3 and Dlx5, whereas osteogenic stimuli enhanced the expression of Dlx3 and Dlx5 in 3T3-L1 preadipocytes. These results suggest that Dlx3 and Dlx5 exert a stimulatory effect on osteogenic differentiation of stem cells through the inhibition of adipogenic differentiation as well as direct stimulation.
