Protective Effect of Mouse Nerve Growth Factor on Amikacin -Induced Ototoxicity
10.3969/j.issn.1006-7299.2014.05.013
- VernacularTitle:鼠神经生长因子对丁胺卡那霉素耳毒性的保护作用
- Author:
Qinfang CAI
;
Lixin JIANG
- Publication Type:Journal Article
- Keywords:
Nerve growth factor;
Amikacin;
Ototoxicity;
Hair cells
- From:
Journal of Audiology and Speech Pathology
2014;(5):507-509
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of mouse nerve growth factor on amikacin (AK)-induced ototoxicity in guinea pigs .Methods 45 guinea pigs were randomly divided into three groups with 15 guinea pigs in each group .The control group received no drug treatment ,the poisoning group received an intraperitoneal injection of AK (400 mg · kg -1 · d-1 ) for 10 days ,and the treatment group received an intraperitoneal injection of AK (400 mg · kg -1 · d-1 ) for 10 days and an intramuscular injection of NGF (1 500 AU · kg -1 · d-1 ) for 14 consecutive days .Auditory brainstem responses were tested on the day before administration ,the 10th day and 20th day after administration .All the guinea pigs were sacrificed when the last ABR test was finished on the 20th day whose Corti organs were observed as well .Results The ABR thresholds of each group showed no significant difference before administration .The ABR threshold of the treatment group was lower than that of the poisoning group .With light microscopy ,the hair cells with normal morphology were observed in the control group .However ,there was an ex-tensive loss of outer hair cell in the poisoning group .For the treatment group ,the miss rate of hair cell was lower compared with the poisoning group .Conclusion Our data shows that NGF has played a protective role on amikacin-induced ototoxicity .
