MK-2206, an inhibitor of Akt, induced cell apoptosis and autophagy in U2 OS cells
10.3969/j.issn.1000-4718.2014.09.007
- VernacularTitle:Akt激酶抑制剂MK-2206诱导U2OS人骨肉瘤细胞凋亡与自噬
- Author:
Xueying WANG
;
Zhaomei LI
;
Yunsheng ZHOU
;
Wenli GUO
;
Fengze WANG
- Publication Type:Journal Article
- Keywords:
MK-2206;
Apoptosis;
Cell autophagy;
U2OS cells
- From:
Chinese Journal of Pathophysiology
2014;(9):1580-1583
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the effect of MK-2206, an inhibitor of Akt, on the cell apoptosis and autophagy of U2OS cells.METHODS:The cell viability was detected by MTT assay .The cell apoptosis was analyzed by TdT-media-ted dUTP nick end labeling assay .The expression of LC3-II was examined by Western blotting .RESULTS:MK-2206 in-hibited the cell viability in a dose-dependent manner .MK-2206 induced the cell apoptosis via activation of caspase-3, caspase-9 and PARP.MK-2206 treatment substantially induced the U 2OS cell autophagy by increasing in the levels of LC 3-II.Blockage of autophagy using chloroquine magnified MK-2206-induced cell death in U2OS cells.CONCLUSION:The Akt inhibitor MK-2206 induces cell apoptosis and autophagy .Blocking autophagy magnifies MK-2206-induced the inhibi-tion of the viability in U2OS cells.
