Wnt signaling pathway is involved in catalpol-induced proliferation of rat bone marrow mesenchymal stem cells
10.3969/j.issn.1000-4718.2014.09.019
- VernacularTitle:梓醇促进大鼠骨髓间充质干细胞增殖过程中W nt信号通路的变化
- Author:
Shuping FU
;
Li YANG
;
Hao HONG
;
Chen OU
;
Ronghua ZHANG
- Publication Type:Journal Article
- Keywords:
Catalpol;
Bone marrow mesenchymal stem cells;
Cell proliferation;
Wnt signaling pathway
- From:
Chinese Journal of Pathophysiology
2014;(9):1656-1660
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the changes of Wnt signaling pathway in catalpol-induced proliferation of rat bone marrow mesenchymal stem cells (BMSCs).METHODS:The BMSCs were isolated from SD rats , purified by differ-ential time adherent method and divided into control group and catalpol (1.0 mg/L) group.Flow cytometry was used to de-tect the proliferation index of BMSCs .The mRNA levels of Wnt3a, Wnt5a, Wnt11 and β-catenin was evaluated by real-time PCR.In addition, the protein expression level of β-catenin was determined by Western blotting .RESULTS:Prolife-ration index was increased from 8.90%±0.46% to 17.93%±1.68% after treatment with catalpol (P<0.01).Com-pared with control group , the mRNA expression of Wnt5a, Wnt11 andβ-catenin was all increased with catalpol treatment . No difference of Wnt3a mRNA expression between control group and catalpol group was observed .Meanwhile, the protein expression of β-catenin was increased in catalpol group compared with control group .CONCLUSION:Catalpol promotes BMSCs going into the cell cycle .Classical and non-classical Wnt signaling pathways are activated in this process .
