Effects of glutamine pretreatment on intestinal ischemia-reperfusion inju-ry in rats
10.3969/j.issn.1000-4718.2014.09.028
- VernacularTitle:谷氨酰胺对大鼠肠缺血再灌注损伤的作用
- Author:
Aili WANG
;
Qiong NIU
;
Chengxia LIU
;
Xingfang JIA
;
Haifeng LIAN
- Publication Type:Journal Article
- Keywords:
Glutamine;
Intestinal ischemia-reperfusion injury;
Oxidative stress
- From:
Chinese Journal of Pathophysiology
2014;(9):1703-1707
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To determine the effects of glutamine ( Gln) pretreatment on intestinal ischemia-reperfusion (I/R) injury in the rats.METHODS: Thirty male Wistar rats were randomly divided into 3 groups (n=10): sham group, I/R group and Gln pretreatment group .The rats in Gln pretreatment group were pretreated with 1 g· kg -1 · d-1 Gln by orogastric route for 7 d, the rats in the other 2 groups were pretreated with normal saline .Intestinal I/R was induced by 30-min occlusion of the superior mesenteric artery followed by 24 h of reperfusion .After the operation , the plasma endo-toxin, serum D-lactic acid, superoxide dismutase ( SOD) and malondialdehyde ( MDA) levels were measured .The intesti-nal mucosal injury was observed with HE staining and evaluated using Chiu 's scoring.RESULTS: Serum D-lactic acid, endotoxin level , MDA level and Chiu's score in I/R group were significantly higher than those in sham group and Gln group (all P<0.05).Serum SOD activity was significantly lower than that in sham group and Gln group (P<0.05).CON-CLUSION:Glutamine has a protective effect on the intestines during ischemia-reperfusion injury .The mechanism may be related to oxidative stress response .
