The biological effect of Survivin siRNA on esoPhageal carcinoma cell line by increasing targeting CasPase-9
l0.3760/cma.j.issn.l008-63l5.20l4.l3.0l0
- VernacularTitle:Survivin siRNA上调Caspase-9蛋白表达影响食管癌细胞的生物学效应
- Author:
Dawei EUI
;
Guogui SUN
;
Shuhai SHENG
- Publication Type:Journal Article
- Keywords:
Survivin;
EsophaGeal carcinoma;
Reverse transcription polymerase chain reaction;
Western blot;
Immunohistochemistry
- From:
Clinical Medicine of China
2014;(z1):26-28
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investiGate the bioloGical effect of inhibitor of apoptosis proteins( Survivin) on esophaGeal cancer ECA-l09 cell line. Methods Survivin siRNA was transiently transfected into non small esophaGeal cancer ECA-l09 cell line by liposome-mediated method and the expression level of Survivin siRNA was detected by RT-PCR and western blot. MTT assay,cell apoptosis,cell cycle and western blot were conducted as to observe proliferation,apoptosis,cycle of cell and expression of Caspase-9. Results RT-PCR and Western blot showed that ECA-l09 cell transfected Survivin siRNA had a lower relative expressive content compared with untransfected Group( P <0. 05 ). MTT assay,cell apoptosis and cell cycles demonstrated that ECA-l09 cell transfected Survivin siRNA had a lower survival fraction,hiGher cell apoptosis,more percentaGe of the G2/M phases( P < 0. 05 ). Conclusion Survivin involve in the bioloGical processes of esophaGeal cancer cell proliferation,apoptosis,and cell cycle.
