Cyclophosphamide intervention in vivo increases the ABCG2 expression in adrenocortical carcinoma cells
- VernacularTitle:环磷酰胺体内干预对肾上腺皮质癌细胞 ABCG2表达的影响
- Author:
Xiaozhou CHEN
;
Zuojie LUO
;
Xiaocong KUANG
;
Yingfen QIN
;
Wenqing ZENG
;
Zhenxing HUANG
;
Yuan QIN
;
Fengping WU
- Publication Type:Journal Article
- Keywords:
ATP-binding cassette super family G member 2;
Adrenocortical carcinoma;
Cyclophosphamide;
Drug resistance
- From:
Journal of Medical Postgraduates
2014;(9):915-917
- CountryChina
- Language:Chinese
-
Abstract:
Objective High expression of multi-resistant transporter ATP-binding cassette super family G member 2 (ABCG2) is a major cause of drug resistance and chemotherapeutic failure of cancer .This study was to investigate the significance of ABCG2 expression in adrenocortical cancer cells after cyclophosphamide ( CTX) intervention in vivo . Methods Ten male and fe-male BALB/C-nu mice were randomly divided into a cyclophosphamide ( CTX) group and a control of equal number .SW-13 cells were subcutaneously injected into the nude mice to establish a model of subcutaneous transplantation tumor , followed by intraperitoneal injec-tion of CTX and isotonic saline solution into the two groups of mice , respectively .Then the expression of ABCG 2 in tumor tissue and primarily cultured cells was detected by immunohistochemistry and flow cytometry . Results The expression of ABCG 2 in the tumor tissue was significantly higher in the CTX than in the control group ([69.1 ±1.83]%vs [53.4 ±1.65]%, P<0.05), and so was that in the primarily cultured cells ([97.89 ±1.36]% vs [81.88 ±8.31]%, P<0.05). Conclusion The ABCG2 gene is in-volved in the drug resistance of adrenocortical carcinoma and may be a therapeutic target of the malignancy .
