Simultaneous Determination of Icariin and Naringin in Bushen Huayu Extract by HPLC

VernacularTitle:高效液相色谱法同时测定补肾化瘀浸膏中淫羊藿苷及柚皮苷含量
Author: Lu OUYANG ; Yong XIA ; Peng XIA ; Hongliang LI ; Xuanbin WANG
Publication Type:Journal Article
Keywords: Bushen huayu extract; Icariin; Naringin; Chromatography,high performance liquid
From: Herald of Medicine 2014;(9):1224-1226
CountryChina
Language:Chinese
Abstract: Objective To establish a method for simultaneous determination of icariin and naringin content in bushen huayu extract by HPLC. Methods Reverse-phase high performance liquid chromatography ( RP-HPLC ) separation was performed one C18 column (4.6 mmí250 mm,5 μm).The mobile phase was acetonitrile containing 0.2%H4PO3(pH=2.9). Gradient elution with a flow rate of 0. 8 mL·min-1 was applied to achieve the separation. The detection wavelength was set at 269 nm,and the column temperature was 30℃. Results Icariin had a good linear range from 0. 3-3. 0μg (r=0. 999 9),the recovery rate was 105. 6%,and RSD was 0. 95%. Naringin was linear within the range of 0. 12-1. 20 μg (r=0. 999 9). The recovery rate was 94. 0%and RSD was 0. 52%. Conclusion The method is simple,stable,accurate,and reproducible,which can be used as the quality control standard for bushen huayue extract.