Effect of Zinc finger protein 185 on the migration of human glioma cells

VernacularTitle:锌指蛋白185对人胶质瘤细胞迁移的影响
Author: Bin LU ; Quanhui ZHENG
Publication Type:Journal Article
Keywords: zinc finger protein; glioma; cell migration
From: Chinese Journal of Clinical Oncology 2014;(17):1084-1088
CountryChina
Language:Chinese
Abstract: Objective:To explore the effect of Zinc finger protein 185 (ZNF185) on the migration of human gliocytoma cells. Methods: ZNF185 protein expression in human gliocytoma and its adjacent normal tissues was detected through Western blot. The ZNF185 gene was cloned and transfected into the human gliocytoma cell line SF767. ZNF185 cells were located through immunofluo-rescence staining, and cell migration was analyzed using the cell scoring method. Results:CZNF185 protein expression was lower in the human gliocytoma cell line than in normal para-neoplastic tissues. ZNF185 protein was mainly expressed in the cytoplasm and cell membrane of the human gliocytoma cells and was co-located with F-actin. ZNF185 overexpression repressed the migration of human gliocytoma cells. Conclusion:ZNF185 represses the migration of human gliocytoma cells by binding to F-actin.