CKLF1 induces SH-SY5 Y cell migration via PLCγ/FAK signaling pathway

VernacularTitle:CKLF1通过激活PLCγ/FAK信号途径促进SH-SY5 Y细胞迁移
Author: Zhenzhen WANG ; Yuhe YUAN ; Ning HAN ; Yi ZHANG ; Naihong CHEN
Publication Type:Journal Article
Keywords: CKLF1; cell migration; FAK; PLCγ; CELLocate; SH-SY5 Y
From: Chinese Pharmacological Bulletin 2014;(9):1209-1213
CountryChina
Language:Chinese
Abstract: Aim To investigate the role of chemokine-like factor 1 ( CKLF1 ) in SH-SY5 Y cell migration and its molecular regulatory mechanism. Methods SH-SY5Y cells were stimulated with CKLF1 for 0. 5 h, 2 h, 8 h and 24 h, respectively. The migration distance and the percentage of migration cells were recorded by CELLocate analysis. The phosphorylation of focal ad-hesion kinase ( FAK) at Tyr-397 site was detected by Western blot analysis. By chemotaxis assays, we con-firmed the chemotaxis of CKLF1. Furthermore, FAK inhibitor PF-573228 and PLCγ inhibitor U73122 were used for the research of molecular regulatory mecha-nisms involved. Results CKLF1 promoted cell migra-tion and induced a strong increase in the phosphoryla-tion level of FAK-pY397 , which were significantly at-tenuated by the presence of U73122 ( a specific inhibi-tor for PLCγ) . In addition, the chemotaxis of CKLF1 was obviously blocked by the FAK inhibitor PF-573228 . Conclusion CKLF1 induces SH-SY5 Y cell migration via PLCγ/FAK signaling pathway.