The protective effects of rTsP38 on intestinal ischemia/reperfusion injury in mice and its underlying mechanisms
10.3969/j.issn.1001-1978.2014.09.012
- VernacularTitle:重组旋毛虫蛋白rTsP38对小鼠肠缺血/再灌注肠损伤的保护作用及机制研究
- Author:
Weifeng LIU
;
Shihong WEN
;
Yunsheng LI
;
Jiantong SHEN
;
Kexuan LIU
- Publication Type:Journal Article
- Keywords:
reperfusion injury;
macrophages;
intes-tine;
inflammation;
neutrophils infiltration;
Trichinella spiralis
- From:
Chinese Pharmacological Bulletin
2014;(9):1235-1241
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the the protective effects of a novel recombinant Trichinella spiralis 38 ku protein ( rTsP38 ) on intestinal I/R injury and the po-tential mechanisms. Methods Male BALB/c mice were randomly divided into sham group ( group S) , in-jury group ( group I) , rTsP38 vaccinated group ( group T) and adjuvants vaccinated group ( group A ) , and received subcutaneously phosphate buffer solution (PBS), PBS, rTsP38, or adjuvants, respectively, at 2-week intervals 6 weeks before the surgical proce-dure. Results Intestinal I/R caused severe intestinal injury evidenced by significant increases in modified Chiu 's score and neutrophils infiltration, accompanied by decreases in daily food intake and body weight. The mRNA level of arginase-1 ( Arg-1 ) was decreased and the mRNA level of inducible nitric oxide synthase 2 ( NOS2) was increased in group I. RTsP38 significant-ly ameliorated intestinal injury and improved intestinal function following intestinal I/R accompanied by de-crease in neutrophils infiltration and increase in cell proliferation in the intestine, compared to mice without rTsP38 pretreatment. Fold changes of Arg-1 mRNA level were significantly increased in group T. Conclu-sions These findings indicate that rTsP38 exerts pro-tection on intestinal I/R injury in mice via promoting M2 macrophages polarization.
