Effect of 3-n-butylphthalide on expression of matrix metalloproteinase andβ-APP in rats
10.3969/j.issn.1009-0126.2014.09.023
- VernacularTitle:丁苯酞对大鼠基质金属蛋白酶及淀粉样前体蛋白表达的影响
- Author:
Wei WEI
;
Ming ZHAO
;
Weiwei ZHANG
- Publication Type:Journal Article
- Keywords:
matrix metalloproteinase 2;
matrix metalloproteinase 9;
amyloid beta-protein precur-sor;
brain ischemia
- From:
Chinese Journal of Geriatric Heart Brain and Vessel Diseases
2014;(9):975-977
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of 3-n-butylphthalide on expression of MMP-2 ,MMP-9 andβ-APP in rats following chronic cerebral hypoperfusion at molecular level .Methods Seventy-two male Wistar rats were randomly divided into sham operation group ,ischemic group ,and bu-tylphthalide group (24 in each group) .Rats in ischemic group and butylphthalide group were di-vided into postischemic 7 ,14 ,30 ,60 d subgroups (6 in each group) .Expressions of β-APP ,MMP-2 , MMP-9 in each group were detected by Western blot .Results The β-APP expression level was significantly higher in postischemic 14 d ,30 d ,60 d subgroups than in postischemic 7 d subgroup (P<0 .05 ,P<0 .01) ,and in postischemic 30 d ,60 d subgroups than in postischemic 14 d sub-group (P< 0 .01) .The β-APP expression level differed greatly in 30 d and 60 d butylphthalide subgroups (P<0 .01) .The MMP-2 expression level was significantly higher in postischemic 60 d subgroup than in postischemic 30 d subgroup (P<0 .05 ,P<0 .01) .Theβ-APP and MMP-2 ex-pression levels were significantly higher in ischemic group and butylphthalide group than in sham operation group (P<0 .05 ,P<0 .01) ,and significantly lower in butylphthalide group than in is-chemic group (P< 0 .01) .Conclusion MMP-2 and β-APP tend to change similarly and are in-volved in blood brain barrier destruction and amyloid deposition .Butylphthalide intervenes in am-yloid deposition by downregulating the expression of MMP-2 andβ-APP .
