Antisense oligonucleotides targeting seed sequence of miR-155 and its ap-plications in multiple myeloma
10.3969/j.issn.1000-4718.2014.08.005
- VernacularTitle:m iR-155种子序列的反义寡核苷酸对抗多发性骨髓瘤的作用
- Author:
Maoxiao FENG
;
Rongxuan ZHU
;
Xiaochuang LUO
;
Chunming GU
;
Xue ZENG
;
Jia FEI
- Publication Type:Journal Article
- Keywords:
miR-155;
Multiplemyeloma;
t-antimiR-155;
RPMI-8266cells;
Oligonucleotides,antisense
- From:
Chinese Journal of Pathophysiology
2014;(8):1368-1373
- CountryChina
- Language:Chinese
-
Abstract:
[ABSTRACT]AIM:ToinvestigatetheroleoftinyantisensenucleicacidagainstmiR-155(tinyantimiR-155, t-antimiR-155) in multiple myeloma cells .METHODS:According to the seed sequence of miR-155, t-antimiR-155 was designed and synthesized .t-antimiR-155 was transfected by Lipofectamine TM 2000 into RPMI-8266 cells.The cells were di-vided into t-antimiR-155 group, scrambled control (SCR) group and blank control group .The growth-inhibitory potencies were measured by MTT assay .The ability of cell colony formation was detected by cell colony formation assay .The cell ap-optosis was assessed by flow cytometry with annexin V /PI double staining .RESULTS: The best concentration and time were 0.4 μmol/L and 48 h, respectively.The cell colony forming experiment showed that the circumstances of forming cell community in t-antimiR-155 group was weaker than that in SCR group , and the colony formation inhibitory rate of former was significant higher than the latter .Compared with SCR group , the cell apoptosis in t-antimiR-155 group significantly in-creased.CONCLUSION: The t-antimiR-155 inhibits the progression of multiple myeloma cells by interfering with miR-155.miR-155 may serve as a potential target in gene therapy for treating multiple myeloma .
