Effects of phospholipid transfer protein overexpression on content of sphingosine-1-phosphate in mouse lipoprotein
10.3969/j.issn.1000-4718.2014.08.015
- VernacularTitle:过表达磷脂转运蛋白对小鼠脂蛋白上鞘氨醇-1-磷酸含量的影响
- Author:
Yang YU
;
Yumei FENG
;
Shoudong GUO
;
Yingjie CUI
;
Guohua SONG
;
Lei FENG
;
Tian LUO
;
Chao CHEN
;
Yiwei WANG
;
Xiancheng JIANG
;
Shucun QIN
- Publication Type:Journal Article
- Keywords:
Sphingosine-1-phosphate;
Phospholipid transfer proteins;
Lipoproteins,HDL;
Apolipoprotein M;
Erythrocytes
- From:
Chinese Journal of Pathophysiology
2014;(8):1421-1426
- CountryChina
- Language:Chinese
-
Abstract:
[ABSTRACT]AIM:Toinvestigatetheinteractionandthemechanismofsphingosine-1-phosphate(S1P)and phospholipid transfer protein (PLTP) in lipoprotein.METHODS:The S1P content in the plasma and lipoprotein from 10-week-old PLTP transgenic (PLTP-Tg) mice and wild-type (WT) mice (n=8 each) was assayed.The transport of S1P by PLTP was determined by S1P transfer assay.The content of specific S1P carrier, apolipoprotein M, was detected by West-ern blotting.RESULTS:Plasma S1P contents were decreased by 21.1%in PLTP-Tg mice compared with WT mice.S1P content in high-density lipoprotein ( HDL) fraction ( HDL-S1P) from PLTP-Tg mice was decreased by 35.1% compared with WT mice, whereas the S1P in low-density lipoprotein (LDL) fraction (LDL-S1P) was increased by 127.4%.The re-sults of S1P transfer assay indicated that PLTP facilitated S 1P transport from erythrocyte to recombinant liposome at 37℃in D-Hanks buffer solution .The plasma content of apolipoprotein M was not changed in PLTP-Tg mice compared with WT mice.CONCLUSION:PLTP is a key factor to maintain plasma HDL-S1P under physical condition .Overexpression of PLTP decreases the HDL-S1P but increases LDL-S1P.The mechanism might be related to the capability of PLTP on trans-ferring S1P from erythrocyte to lipoprotein.
