Effect of acid sensing ion channels on respiratory regulation by central chemoreceptors
10.3969/j.issn.1000-4718.2014.08.011
- VernacularTitle:酸敏感离子通道在中枢化学感受器呼吸调节中的作用
- Author:
Li LI
;
Wenyan LIU
;
Bo GAO
- Publication Type:Journal Article
- Keywords:
Acidsensingionchannels;
Respiratoryregulation;
Centralchemoreceptor
- From:
Chinese Journal of Pathophysiology
2014;(8):1400-1403
- CountryChina
- Language:Chinese
-
Abstract:
[ABSTRACT]AIM:Toinvestigatetheeffectofintracerebroventricular(icv)injectionofacidulatedartificialce-rebrospinal fluid ( aCSF ) on the respiratory reactions and the functions of acid sensing ion channels ( ASICs ) in this process.METHODS:Healthy adult SD rats (n=30) were divided into aCSF with pH 7.4 control group, aCSF with pH 6.5 group, amiloride control group, amiloride plus aCSF with pH 6.5 group, psalmotoxin 1 (PcTx1) control group and PcTx1 plus aCSF with pH 6.5 group.The electromyogram (EMG) of the diaphragm was monitored to observe the respirato-ry responses induced by icv injection of acidulated aCSF .The ASICs blockers were also injected into the lateral cerebral ventricle firstly and acidulated aCSF was injected following the ASICs blockers to observe the effect of ASICs on the respira -tory regulation by the central chemoreceptor .RESULTS:After icv injection of acidulated aCSF , the respiratory responses of the rats were excited (P<0.05).The respiratory excitation responses disappeared after icv injection of ASICs blocker amiloride.The degree of respiratory excitation was weakened after icv injection of ASIC 1a blockers-PcTx1.CONCLU-SION:ASICs play a key role in the respiratory regulation by the central chemoreceptor and ASIC 1a partly operates in this process.
