Silencing IDH-2 gene by siRNA-IDH-2 inhibits human small cell lung car-cinoma growth
10.3969/j.issn.1000-4718.2014.08.008
- VernacularTitle:干扰 ID H-2基因对小细胞肺癌生长抑制作用的研究
- Author:
Jianhong LU
;
Guojun CHEN
;
Changlin DONG
;
Shaowen GUO
;
Yijun JIN
- Publication Type:Journal Article
- Keywords:
Isocitratedehydrogenase2;
RNAinterference;
Lungneoplasms
- From:
Chinese Journal of Pathophysiology
2014;(8):1384-1387
- CountryChina
- Language:Chinese
-
Abstract:
[ABSTRACT]AIM:Toinvestigatetheeffectofsilencingisocitratedehydrogenase2(IDH-2)genebysmallinter-fering RNA (siRNA) on the biological characteristics of human small cell lung cancer cell line NCI -H446.METHODS:IDH-2 expression was knocked down in human small cell lung cancer cell line NCI -H446 by siRNA-IDH-2.The expression level of IDH-2 was determined by real-time PCR and Western blotting .The cell proliferation was measured by CCK-8 as-say , the protein expression of MAPK p 42 was detected by Western blotting , and the cell cycle was analyzed by flow cytome-try.The migration was observed using Transwell cell migration system .BALB/c nude mice were subcutaneously injected on the back with NCI-H446 cells transfected with siRNA-IDH-2/negative control siRNA or non-transfected cells to study the tumor growth .RESULTS:siRNA-IDH-2 remarkably down-regulated the expression of IDH-2 and MAPK p42 in the NCI-H446 cells.siRNA-IDH-2 inhibited both the proliferation and migration abilities of NCI-H446 cells, and the cell cycle was arrested in S phase as compared with negative control group .Additionally, the volume of xenograft tumors in siRNA-IDH-2 group was significantly decreased as compared with control group .CONCLUSION:siRNA-IDH-2 down-regulates the expres-sion of IDH-2 in NCI-H446 cells, reduces the cell migration efficiency and inhibits the tumor growth in vitro and in vivo.
