Farnesoid X receptor up-regulates thyrotropin embryonic factor and at-tenuates pathological injury of Con A-induced hepatitis

VernacularTitle:FXR 通过调节促甲状腺素胚胎因子减轻 Con A诱导的自身免疫性肝炎病变
Author: Fan LIAN ; Yu WANG ; Jiaping LI ; Xiwen WU ; Juncong XIE ; Zeshen WU ; Guanqi LIU ; Hanshi XU ; Liuqin LIANG ; Xiuyan YANG ; Jianyong YANG
Publication Type:Journal Article
Keywords: Farnesoid Xreceptor; Thyrotropin embryonic factor; Hepatitis,autoimmune
From: Chinese Journal of Pathophysiology 2014;(8):1445-1450
CountryChina
Language:Chinese
Abstract: [ABSTRACT]AIM:ToobservehowfarnesoidXreceptor(FXR)functionedinconcanavalinA(ConA)-induced hepatitis (CIH) and the regulation of FXR-thyrotropin embryonic factor (TEF) pathway.METHODS:C57BL/6 mice were injected with Con A to induce hepatitis .The expression of FXR and TEF in the liver specimens was determined by qRT-PCR and Western blotting .The concentrations of serum ALT/AST and inflammatory cytokines IFN-γ, TNF-α, IL-4 and IL-2 in the blood samples were tested after Con A injection .RESULTS:FXR was down-regulated in CIH mice .TEF was up-regula-ted when FXR was activated by chenodeoxycholic acid (CDCA).Activation of FXR reduced the levels of aminotransferases and inflammatory cytokines IFN-γ, TNF-α, IL-4 and IL-2 in the CIH mice induced by Con A injection .CONCLUSION:FXR activation attenuates CIH mouse liver injury and reduces inflammatory cytokines .FXR activation results in TEF up-regu-lation.The FXR-TEF pathway may play a protective role in autoimmune hepatitis .