DHA promotes NGF-induced neuronal differentiation in PC12 cells via activating BMP pathway

VernacularTitle:DHA促NGF诱导的PC12细胞分化与BMPs通路关系的研究
Author: Xin ZHOU ; Baoyan SHI ; Kefeng WU ; Xiang GAO ; Junyan HUANG ; Ren HUANG ; Wende LI
Publication Type:Journal Article
Keywords: DHA; BMP pathway; PC12 cell; NGF; differentiation; neurite
From: Chinese Pharmacological Bulletin 2014;(9):1247-1251
CountryChina
Language:Chinese
Abstract: Aim To investigate the effect of DHA on NGF-induced neuronal differentiation of PC12 cells and explore the possible mechanism via regulating BMP pathway. Methods PC12 cells were treated with 100μg·L-1 NGF and 100 μg·L-1 NGF + 10 μmol· L-1 DHA for 3, 6 and 9 days respectively. The length and number of neurite were detected by immunofluores-cenc. DHA content was analyzed by gas chromatogra-phy in all groups. The protein expression of BMP4, BMP7 , BMPR-II and p-Smad 1/5/8 was determined by Western blot. Results The length of total primary neurite in NGF+DHA groups was obviously increased, longer than that in NGF group; DHA content in 10μmol · L-1 DHA group was higher than that in the control group;NGF+DHA groups also unregulated the protein expression of BMP4 , BMP7 , BMPR-II and p-Smad 1/5/8 . Conclusion DHA promotes NGF-in-duced neuronal differentiation in PC12 cells, which may be associated with the upregulation of BMP path-way protein.