Effects of salicylate on voltage-gated calcium channels in rat hippocampal neurons
10.3969/j.issn.1001-1978.2014.09.018
- VernacularTitle:水杨酸钠对大鼠海马神经元电压门控性钙离子通道的影响
- Author:
Xiaoli ZHU
;
Yanxing LIU
;
Baogui ZHONG
;
Hongfang MA
;
Ping JIANG
;
Xiaoyan ZHANG
- Publication Type:Journal Article
- Keywords:
salicylate;
tinnitus;
hippocampal neu-rons;
voltage-gated calcium channels;
whole-cell patch-clamp technique;
rat
- From:
Chinese Pharmacological Bulletin
2014;(9):1266-1270
- CountryChina
- Language:Chinese
-
Abstract:
Aim To provide an electrophysiological basis for hippocampus involved emotional changes caused by tinnitus, the effects of salicylate on electro-physiological characteristics of the voltage-gated calci-um channels in hippocampal neurons were performed. Method The effects of salicylate on voltage-gated cal-cium channels in rat hippocampal neurons were stud-ied, using the whole-cell voltage clamp method. Re-sults Salicylate blocked the voltage-gated calcium channels ( ICa ) in a concentration-dependent manner (0.1~10 mmol · L-1 ) . The half-inhibition concen-tration ( IC50 ) values for the blocking action of salicy-late on ICa were 1.64 mmol·L-1 . With 1 mmol·L-1 salicylate applicalted into bath solution, the steady-state activation curve of calcium channel was shifted by about 9 mV in the hyperpolarizing direction, and its steady-state inactivation curve was not changed. Con-clusion Salicylate inhibits ICa in rat hippocampal neu-rons and significantly affects the activation kinetics of ICa ,which could be related to emotional changes caused by tinnitus.
