Lentivirus mediated CCN1 gene on growth and migration of rat bone marrow mesenchymal stem cells

VernacularTitle:慢病毒介导的 CCN1基因对大鼠骨髓间充质干细胞生长和迁移的影响
Author: Zhan SUN ; Xueli GONG ; Xinjian RAN ; Qi MA ; Mei LONG
Publication Type:Journal Article
Keywords: CCN1 protein; Bone marrow mesenchymal stem cells; Lentivirus
From: Chinese Journal of Pathophysiology 2014;(8):1493-1496
CountryChina
Language:Chinese
Abstract: [ABSTRACT]AIM:Toinvestigatetheroleofcysteine-rich61(Cyr61/CNN1)inproliferationandmigrationof bone marrow mesenchymal stem cells ( BMSCs ) .METHODS: The lentiviral vector carrying CCN 1 ( Lenti-GFP-CCN1 ) was constructed and then transfected into the rat BMSCs .The cells were divided into non-transfection group , transfection group ( transfected with Lenti-GFP-CCN1 ) and negative control group ( Lenti-GFP ) .The fluorescence intensity of the transfected BMSCs was observed under inverted fluorescence microscope .The effects of CCN1 on the proliferation and mi-gration of BMSCs were detected by MTT assay and scratch wound healing assay .RESULTS:The proliferation of BMSCs transfected with Lenti-GFP CCN1 had no significant difference compared with negative control group and control group .The width/thickness ratio of migrated BMSCs in wound healing was significantly higher in Lenti-GFP-CCN1 group than that in negative control group and control group (P<0.05).CONCLUSION:Exogenous CCN1 promotes the migration of BMSCs.