Establishment and evaluation of isoprenaline-induced myocardial fibrosis mouse model
10.3969/j.issn.1001-1978.2014.09.030
- VernacularTitle:异丙肾上腺素致小鼠心肌纤维化模型的制备及评价
- Author:
Rong CHEN
;
Meilin XIE
- Publication Type:Journal Article
- Keywords:
isoprenaline;
myocardial fibrosis;
mouse model;
car-diac weight index;
hydroxyproline;
collagenI
- From:
Chinese Pharmacological Bulletin
2014;(9):1325-1328
- CountryChina
- Language:Chinese
-
Abstract:
Aim To establish and evaluate a mouse model of my-ocardial fibrosis by hypodermic injection of isoprenaline. Meth-ods Kunming mice were randomly divided into 2 groups, namely, the myocardial fibrosis model group and the control group, 10 mice in each group. The mice in the model group were given isoprenaline 5 mg · kg-1 by hypodermic injection. From the following day, the dose of isoprenaline was reduced to 2. 5 mg·kg-1 , and lasted for 30 days. The mice in the control group were treated with the physiological saline in the same way. Final-ly, heart weight was then weighed and the cardiac weight index (CWI) was calculated. Hydroxyproline (Hydro) level in myo-cardium was determined by a colorimetric method. HE and Mas-son’s trichrome staining were used to estimate the extent of myo-cardial fibrosis and calculate the collagen volume fraction ( CVF) . RT-PCR was used to measure the myocardial Collagen I mRNA expression. Results Compared with the control group, the CWI and Hydro content in the myocardial tissues in the model group were increased(P<0.01). The content of col-lagen in the myocardial tissues and the CVF were increased obvi-ously(P<0.01). The RT-PCR results showed that the left ven-tricle Collagen I mRNA expression in the model group increased obviously( P<0.01 ) . Conclusion Isoprenaline-induced myo-cardial fibrosis model has been established and the method is very simple, economic and reliable.
