Identification of the expression of recombinated plasmid pLNCX-EGFP-C1-hri in PA317 packaging cell line
10.3969/J.ISSN.1672-8270.2014.05.004
- VernacularTitle:增强型绿色荧光蛋白融合人核糖核酸酶抑制因子包装细胞系的建立及鉴定
- Author:
Ning DING
;
Kun LI
;
Ming CHANG
- Publication Type:Journal Article
- Keywords:
Human ribonuclease inhibitor;
Enhanced green fluorescent protein;
Identification
- From:
China Medical Equipment
2014;(9):9-11
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To identify the expression of plasmid pLNCX-EGFP-C1-hri targeting the gene of Human ribonuclease inhibitor (hri) in PA317 cells which is capable of expression in mammalian cells.Methods: The vector of pLNCX-EGFP-C1-hri was transfected into PA317 cells by Lipofectamine 2000 and then the expression of recombinated plasmid was verified in living cells by observing the transcription level of egfp-hri fusion gene mRNA with RT-PCR method and the expression level of egfp-fusion hRI protein with western blotting method respectively.Results: Both RT-PCR and western blotting showed the egfp-hri fusion gene was obviously expression in PA317 cells.Conclusion: The plasmid of pLNCX-EGFP-C1-hri targeting hRI is successfully constructed and the protein of hRI can be expressed in PA317 cells correctly.
