Zinc-finger BED domain-containing 3 expression in tissues of various organs in normal C57BL/6J mice and the changes in the ZBED3 expression levels affected by liraglutide in C57BL/6J mice and db/db mice
10.3760/cma.j.issn.1000-6699.2014.08.013
- VernacularTitle:锌指蛋白3在普通C57BL/6J小鼠组织器官中的表达差异及利拉鲁肽对其在C57BL/6J和db/db小鼠肝脏表达的影响
- Author:
Hongxia PAN
;
Xianshu JIANG
;
Wenjuan QU
;
Yunsong GUO
;
Gangyi YANG
;
Ling LI
;
Zhiyong LI
- Publication Type:Journal Article
- Keywords:
Zinc-finger BED domain-containing 3;
Glucose;
Insulin;
Liraglutide
- From:
Chinese Journal of Endocrinology and Metabolism
2014;(8):689-692
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare zinc-finger BED domain-containing 3 ( ZBED3 ) expression in various tissues of C57BL/6J mice and the effects of liraglutide, glucose, and insulin on the levels of ZBED3 protein expression in C57BL/6J mice and db/db mice. Methods The mRNA level of ZBED3 in various tissues of C57BL/6J mice was measured by realtime PCR. The protein level of ZBED3 was measured by using western blot. Results ZBED3 mRNA levels were detected in muscle, spleen, kidney, brain, heart, lung, and liver of C57BL/6J mice, yielding the highest expression in muscle. Additionally, The liver ZBED3 levels were higher in db/db mice compared with C57BL/6J mice (P<0. 01). Furthermore, the protein expression of ZBED3 was significantly increased in liver tissues of db/db mice treated with high concentrations of liraglutide, glucose or insulin(P<0. 05), however, the expression of ZBED3 only responded to high concentration of glucose in liver tissues of C57BL/6J mice. Conclusion ZBED3 may act as a novel factor in regulating glucose metabolism. The expression of ZBED3 can be regulated by liraglutide, glucose, and insulin. Thus, ZBED3 may play an important role in conditioning of hyperglycemia.
