Effect of different degrees of intermittent hypoxia on inflammatory cytokines and adipokines in rats
10.3760/cma.j.issn.1000-6699.2014.08.014
- VernacularTitle:不同程度间歇低氧对大鼠炎性细胞因子和脂肪因子的影响
- Author:
Qingchan YANG
;
Hui ZHU
;
Yan WANG
;
Qing HE
;
Jing FENG
;
Baoyuan CHEN
- Publication Type:Journal Article
- Keywords:
Intermittent hypoxia;
Inflammation;
Leptin;
Adiponectin
- From:
Chinese Journal of Endocrinology and Metabolism
2014;(8):693-696
- CountryChina
- Language:Chinese
-
Abstract:
To study the effect of different levels intermittent hypoxia on inflammatory cytokines and adipokines in a rat model. 160 male Wistar rats were divided into five groups, three groups with intermittent hypoxia (5% O2:IH-1 group;7. 5% O2: IH-2 group;10% O2: IH-3 group), continuous hypoxia group (10% O2, CH group);and the intermittent normal oxygen control group(IN). Before the exposure and at the second, fourth, sixth, and eighth week after the exposure, eight rats in each group were selected randomly with blood samples collected. Blood glucose was measured by glucose oxidase-peroxidase ( GOD-POD ) reagents, double sandwich enzyme-linked immunosorbent assay ( ELISA) was used to detect serum concentration of insulin, tumor necrosis factor alpha( TNF)-α, interleukin ( IL)-6, leptin, and adiponectin and adipocyte nuclear factor kappa B ( NF-κB) levels. In IH-1, IH-2, and IH-3 group, blood glucose, insulin, TNF-α, IL-6, leptin, and nuclear NF-κB in rats showed consecutive increment after exposure to intermittent hypoxia, and those findings in IH-1 group were higher than those in IH-2 and IH-3 group (P<0. 05), but the level of TNF-α dropped slightly on the 8th week. The level of adiponectin showed consecutive decrease from the outset and its level in IH-1 group was significantly lower than that in IH-2 and IH-3 group(P<0. 05). Cytoplasmic NF-κB levels did not have obvious difference in all these groups(P>0. 05). These results demonstrate that intermittent hypoxia could activate NF-κB and result in extensive changes of proinflammatory cytokines and adipokines in the serum of rats. The release of pro-inflammatory cytokines and adipokines keeps pace with the degree of intermittent hypoxia.
