HPV E6/E7 mRNA detection applied in the cervical cancer screening in Huludao region
10.3969/j.issn.1673-4130.2014.17.006
- VernacularTitle:HPV E6／E7 mRNA 检测在葫芦岛地区宫颈癌筛查中的应用
- Author:
Li LI
- Publication Type:Journal Article
- Keywords:
E6/E7 mRNA,human papilloma virus;
ultra-thin liquid-based cytology;
cervical cancer screening
- From:
International Journal of Laboratory Medicine
2014;(17):2285-2286
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the human papillomavirus (HPV)E6/E7 mRNA detection used in cervical cancer screen-ing.Methods In the study,160 patients treated in gynaecology department of the hospital were enrolled,whose samples were col-lected for ultra-thin liquid-based cytology(thin prep cytology test,TCT).According to the TCT results,the patients were divided into 4 groups,including normal group(n=32),CINⅠ group(n=44),CINⅡ group(n=67),CINⅢ group(n=17).In addition to the CIN classification,according to the type of pathology the patients′groups were also devided.In different groups,the differences be-tween HPV E6/E7 mRNA and HPV DNA positive rate were compared respectively.The differences between HPV E6/E7 mRNA and HPV DNA levels among different pathology groups were also compared.Results In groups of different CIN grades and control group,the positive rate of HPV E6/E7 mRNA and HPV DNA test showed statistically diffrences (P <0.05 ).Compared among different pathologic groups HPV E6/E7 mRNA and HPV DNA levels were statistically significant (P <0.05).Conclusion In cer-vical cancer screening,HPV E6/E7 mRNA screening can be used as a screening method and its combined use with cytological meth-od would be helpful to improve the diagnostic accuracy.
