Analysis of etiology of bloodstream infection in children with hematologic disease by using PCR
10.3969/j.issn.1673-4130.2014.17.016
- VernacularTitle:PCR 检测在血液病患儿血流感染细菌谱分析中的应用
- Author:
Junhua HUANG
;
Shuwan ZHANG
- Publication Type:Journal Article
- Keywords:
polymerase chain reaction;
bloodstream infection;
bacteria cultivation;
hematopathy;
child
- From:
International Journal of Laboratory Medicine
2014;(17):2307-2308
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the value of PCR for analyzing the pathogenic spectrum of bloodstream infection(BSI)in chil-dren with blood disease,and to provide a new thought for epidemiological survey of BSI.Methods A total of 80 children with blood disease in hematology department with suspected bacterial infections were recruited in the study,whose blood samples were collect-ed and detected by using blood cultivation(BC)and 16 S rDNA-PCR.The results obtained by the two methods were compared.Re-sults Among the 80 children,the positive rate of BC and PCR were 8.8% and 20.0%,respectively.There was significant differ-ence between the two methods(χ2 =5.82,P <0.05).Gram-positive bacteria and Gram-negative bacteria accounted for 68.7% and 31.3% respectively in 16 bacteria.The predominant pathogen was Staphylococcus epidermidis (31.3%).Conclusion Compared with BC,16 S rDNA-PCR and sequencing provide a new way for analyzing the pathogenic spectrum of BSI,which might be effective for epidemiological investigation.The pathogens of BSI are mainly Gram-positive bacteria in children of hematology department,and Staphylococcus epidermidis is the most common pathogen.
