Bioinformatics analysis of lung cancer metastasis-related miR-29b
10.3969/j.issn.1000-8179.20132018
- VernacularTitle:肺癌转移相关microRNA miR-29b的生物信息学分析
- Author:
Hongyan WANG
;
Shaoqiu ZHENG
;
Yongsheng TU
;
Yajie ZHANG
- Publication Type:Journal Article
- Keywords:
miR-29b;
lung cancer;
target gene;
bioinformatics
- From:
Chinese Journal of Clinical Oncology
2014;(16):1021-1025
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This paper aims to bioinformatically analyze the target genes of miR-29b and to provide clues for cancer research targeting miR-29b. Methods:The differential expression levels of miRNAs in CD133+and CD133-A549 cells were detected using the miRNA PCR chip. Real-time polymerase chain reaction was performed to verify the partially differential expression of miR-NAs. Target genes of miR-29b were predicted by miRecords and analyzed by gene ontology and signal transduction pathway enrich-ment analysis. Results: The miR-29b expression was significantly decreased in the CD133+A549 cells compared with that in the CD133-cells. The number of miR-29b target genes was 106. The functions of these target genes were enriched in binding and extracel-lular matrix structural constituent (P<0.01). The JAK-STAT and TGF-βsignal transduction pathways were significantly enriched (P<0.05). Conclusion:The abnormal expression of miR-29b may be related to metastasis. Some of the predicted target genes of miR-29b were significantly enriched in the signaling pathways in relation to the tumors.
