Effects of Total Flavonoids from Portulaca on Transforming Growth Factor β1 in Rats with Hepatic Fibrosis
10.3870/yydb.2014.09.006
- VernacularTitle:马齿苋总黄酮对肝纤维化大鼠转化生长因子-β1信号因子表达的影响
- Author:
Xiaoli PAN
;
Yongai XIONG
;
Yuzhu TAN
;
Hui XIANG
- Publication Type:Journal Article
- Keywords:
Total flavones from portulaca;
Liver cirrhosis;
Transforming growth factors
- From:
Herald of Medicine
2014;(9):1140-1143
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of total flavonoids from portulaca against liver fibrosis in rats by detecting TGF-β1 gene and protein expressions. Methods A total of 48 SD rats were randomly divided into normal control, model control, glucyrrhizin aqueous,and total flavonoids groups,with 12 rats in each group. Except those in the normal control group, rats in other groups were intraperitoneally injected with 2 mL · kg-1 · d-1 carbon tetrachloride to induce liver fibrosis. Rats in glucyrrhizin aqueous group and total flavonoids ones were intragastrically administered with 15. 75 mg · kg-1 of glycyrrhizin aqueous solution or 35. 6 mg·kg-1 of total flavonoids aqueous solution,respectively. The normal and model control groups were administered with equal volume of aqueous solution. Thirty days later,rats were sacrificed by anesthesia. Livers were obtained to detect TGF-β1 gene and protein expressions by RT-PCR and Western-Blot. Results Relative gene expression of TGF-β1 in the normal control,model control,glucyrrhizin aqueous and flavonoids groups was 0. 725±0. 130,7. 493±1. 410,3. 016±1. 240,and 2. 668±1. 150,respectively. Total flavonoids from portulaca significantly reduced the gene (P<0. 01) and protein (P<0. 01) expressions of TGF-β1 . Conclusion Efficacy of total flavonoids from portulaca in treating hepatic fibrosis may be related to decreased TGF-β1 expression in rats.
