Establishment of a severe aplastic anemia mouse model by using recombinant human interferon-gamma plus busulfan
10.3969/j.issn.2095-4344.2014.36.022
- VernacularTitle:重组人γ-干扰素联合白消安诱导建立小鼠重型再生障碍性贫血模型
- Author:
Xiang LIU
;
Shuping ZHONG
;
Lijun HOU
;
Feng XIE
;
Xuegang LI
;
Wenzheng PANG
;
Jingbo XU
;
Zhiguo HE
- Publication Type:Journal Article
- Keywords:
anemia,aplastic;
model,mouse;
interferon-γ
- From:
Chinese Journal of Tissue Engineering Research
2014;(36):5868-5873
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:It is important to establish an ideal mouse model of severe aplastic anemia for investigating the mechanism and finding new therapies for aplastic anemia. OBJECTIVE:To establish a severe aplastic anemia mouse model by using recombinant human interferon-γand busulfan. METHODS:Sixty healthy Kunming female mice were randomly divided into two groups:model group (n=50) and control group (n=10). The model group was given recombinant human interferon-γat a dose of 1×104 U/d by intraperitoneal injection and busulfan at a dose of 18 mg/(kg·d) through stomach feeding for 7 days. The same volume of physiological saline was given to control group. Multi-parameters, including general condition, body weight, blood cellcount, morphology and biopsy of bone marrow were analyzed in two groups. RESULTS AND CONCLUSION:At day 7 after treatment, the weight, white blood cellcount, hemoglobin, blood platelet, reticulocyte count in model group were significantly lower than control group (P<0.05). Bone marrow smears and biopsy of model group showed marked reduction of bone marrow proliferation and increases of percentages of non-hematopoietic cellclusters and adipose tissue. The oil drop and fat vacuole were apparently seen in the model group. Severe aplastic anemia mouse model can be established by using recombinant human interferon-γand busulfan successful y, which is economic, stable and easy to operate.
