Detection of low-concentration HBsAg and its clinical significance
10.3969/j.issn.1673-4130.2014.17.042
- VernacularTitle:低水平 HBsAg 标本的检测及其临床意义
- Author:
Qin LIU
;
Hui PENG
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus surface antigen;
low leve;
clinical value
- From:International Journal of Laboratory Medicine
2014;(17):2364-2366
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the methods for the detection of serum samples with low-concentration HBsAg and its clin-ical value.Methods Serum samples with HBsAg weak positive were measured by ELISA or using Roch cobas e601 were collected from the clinical laboratory.The samples with low-concentration HBsAg were confirmed by neutralization assay by using Lizhu ELISA reagent Kit or Roch cobas e601 analyzer.at the same time,ALT/AST concentrations and HBV DNA copies were also deter-mined.Results Among 53 samples that showed weak positive reaction by using Kehua reagent kit,37 samples were positive in neu-tralization test,while the other 16 samples were negative.Among 18 samples that showed weak positive reaction tested by using Roch cobas e601 analyzer,13 samples showed positive in neutralization test,and the other 5 samples showed negative.Conclusion The results of HBsAg test varies in different detection System.The clinical significance of samples with low-concentration HBsAg needs further study.
