Colorimetric Determination of Lactate Dehydrogenase on Paper-Based Microwell Arrays Microfluidic Device
10.11895/j.issn.0253-3820.140331
- VernacularTitle:纸基微孔阵列芯片比色法检测乳酸脱氢酶
- Author:
Huiyan ZHANG
;
Zhen ZHANG
;
Xinghu JI
;
Zhike HE
- Publication Type:Journal Article
- Keywords:
Lactate dehydrogenase;
Human serum albumin;
Paper-based microwell arrays microfluidic device;
Colorimetric method
- From:
Chinese Journal of Analytical Chemistry
2014;(9):1276-1280
- CountryChina
- Language:Chinese
-
Abstract:
A low-cost, simple, sensitive detection method of lactate dehydrogense ( LDH) was developed on paper-based microwell arrays microfluidic device. The phenazine methyl sulfate/nitrotetrazolium blue chloride ( PMS/NBT) detection system was used for LDH detection and the colorimetric results were recorded by both Gel Documentation System and a common camera. Under the optimized conditions, the colorimetric intensity showed a linear correlation to the activity of LDH in the range of 10 to 150 U/L with a limit of detection (LOD) of 9. 44 U/L (3σ) by Gel Documentation System;and the linear range was 15-150 U/L by camera with a LOD of 12. 36 U/L (3σ). Foremost, it was found that human serum albumin (HSA) had an effect on the colorimetric enhancement in this detection system. This low-cost, portable paper-based analytical platform could be suitable for the application in the point-of-care with high sensitivity and reproducibility.
