Quantitative Determination of Selenium Species in Se-enriched Yeast Using Liquid Chromatography-Hydride Generation Atomic Fluorescence Spectrometry
10.11895/j.issn.0253-3820.140390
- VernacularTitle:液相色谱-氢化物发生原子荧光光谱法测定富硒酵母中硒的形态
- Author:
Zhiming XIAO
;
Rong SONG
;
Zheng JIA
;
Yang LI
;
Xia FAN
- Publication Type:Journal Article
- Keywords:
Liquid chromatography;
Hydride generation atomic fluorescence spectrometry;
Se-enriched yeast;
Selenium species
- From:
Chinese Journal of Analytical Chemistry
2014;(9):1314-1319
- CountryChina
- Language:Chinese
-
Abstract:
A rapid and sensitive method has been developed for the simultaneous determination of four selenium species Se(Ⅵ), Se(Ⅵ), selenomethionine, and Se-methylselenocysteine in Se-enriched yeast by liquid chromatography-hydride generation atomic fluorescence spectrometry (HPLC-HG-AFS). The isolation of the analytes from yeast samples was accomplished by proteaseⅩⅣ and trypsin enzymatic digestion. The target compounds were separated on a PRP-X100 anion exchange column and analyzed by HG-AFS. The mobile phase was 20 mmol/L (NH4)2HPO4. Good linearity was obtained for all the selenium species, with linear correlation coefficients higher than 0. 9996. The LODs of the four species were between 0. 5 and 5. 0 μg/kg. Average recoveries for the four analytes were in the ranges of 82 . 5%-101 . 2%, with intra-and inter-day RSD lower than 8. 6% and 14. 5, respectively. The proposed analytical method is simple, sensitive, with low operation cost, making it applicable for the determination of the selenium species in Se-enriched feeds.
