Application of Modified Mesoporous Silica Materials for Analysis of Small Molecules by MALDI-TOF-MS
10.11895/j.issn.0253-3820.131246
- VernacularTitle:氧化硅介孔材料在基质辅助激光解析飞行时间质谱分析有机小分子中的应用
- Author:
Meihua DONG
;
Shimei SUN
;
Biao JIN
- Publication Type:Journal Article
- Keywords:
Mesoporous Silica;
Small organic molecules;
Matrix-assisted laser desorption/ionization-time of flight-mass spectrometry
- From:
Chinese Journal of Analytical Chemistry
2014;(9):1235-1239
- CountryChina
- Language:Chinese
-
Abstract:
Mesoporous silica SBA-15 with a small organic molecular (1,8-Naphthalimide) was synthesized and used as matrix for MALDI-TOF-MS ( Matrix-assisted laser desorption/ionization time of flight mass spectrometry) analysis. A modified SBA-15 matrix, SBA-15-NA (1,8-Naphthalimide) was synthesized by covalently attaching SBA-15 to an 1 , 8-naphthalimide formed by 1 , 8-naphthalic anhydride and 3-( trimethoxysilyl) propylamine. The mass spectra obtained with SBA-15-NA exhibited less background interference ions, higher desorption/ionization efficiency, and higher peak intensity. The matrix was initially prepared in methanol and mixed with analyte at a 1∶1 volume ratio in vial. The solution of mixture was at a 10∶1 concentration ratio of matrix/analyte on a stainless steel target and allowed to air dry. The method was used to analyze various types of low molecular weight ( less than 500 ) chemical compounds such as monosaccharides, amino acids, phytohormones, drugs. Under the optimal conditions, it gave low detection limit of 1×10-9 g/L (Leucine, S/N=5) and good reproducibility (≤30%). The modified mesoporous silica materials have been employed in the urinary study for direct detection of metabolites.
