Rapid Determination of N-Methylcarbamoyl Adduct in Hemoglobin of Workers Exposed to N,N-Dimethylformamide by Ultra High Performance Liquid Chromatography-Mass Spectrometry
10.11895/j.issn.0253-3820.140282
- VernacularTitle:超高效液相色谱-质谱联用技术快速检测二甲基甲酰胺职业接触者血红蛋白中新型生物标志物
- Author:
Chunmin WANG
;
Qiang LIU
;
Jian LI
;
Bo XU
;
Kai MI
;
Juan CHENG
- Publication Type:Journal Article
- Keywords:
Ultra high performance liquid chromatography-mass spectrometry;
N,N-Dimethylformamide;
N-Methylcarbamoyl adduct;
3-Methyl-5-isopropylhydantoin;
Biomarker
- From:
Chinese Journal of Analytical Chemistry
2014;(9):1326-1331
- CountryChina
- Language:Chinese
-
Abstract:
A rapid analytical method was developed for the determination of N-methylcarbamoyl adduct in Hemoglobin of workers exposed to N,N-dimethylformamide by ultra high performance liquid chromatography-mass spectrometry ( UPLC/MS/MS). About 0. 1 g of hemoglobin sample, 40 μmol/L of 3-methyl-5-isopropylhydantoin (MIH) as the internal standard and 4. 75 mL of HCl-acetic acid (2∶1, V/V) were added in the centrifuge tube, and mixed for 3 min. Then the tube was heated in boiling water bath for 1h. After cooling down, 200 μL of the mixture and 600 μL of formic acid-acetonitrile (1%) were added into 96-well extract plate. The vacuum pump pressure was controlled to make the sample collection elute within 2-4 min.The purified collection was transferred into the sample vial, and 3-methyl-5-isopropylhydantoin ( MVH ) as degration product of N-methylcarbamoyl adduct was quantified by UPLC/MS/MS in multiple reaction monitoring ( MRM ) by internal standard method. A good linear relationship was obtained in the MVH concentration range of 0 . 01-1 . 0 μmol/L with the correlation coefficient of 0 . 999 . The recovery of added MVH in the blank sample was 97 . 3% and the relative standard deviation was 1 . 7%. The limit of detection (LOD) was 0. 01 μmol/g. This method was proved to be fast and efficient.
