Simultaneous Determination of Protocatechualdehyde, Rosmarinic Acid and Salvianolic Acid A in Salvia Miltiorrhiza Bunge by HPLC
- VernacularTitle:HPLC法同时测定丹参中原儿茶醛、迷迭香酸、丹酚酸 A的含量
- Author:
Zhaoyu WANG
;
Yiping XU
;
Rong JIAO
;
Na ZHAO
;
Jiang LI
;
Peng LI
;
Qinhua CHEN
- Publication Type:Journal Article
- Keywords:
Salvia miltiorrhiza Bunge;
Protocatechualdehyde;
Rosmarinic acid;
Salvianolic acid A;
Determination;
HPLC
- From:
China Pharmacist
2014;(9):1473-1475
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method to simultaneously determine the content of protocatechualdehyde, rosmarinic acid and salvianolic acid A in Salvia miltiorrhiza Bunge. Methods: The chromatographic column was a Linksil-ODS ( 250 mm × 4. 6 mm,5 μm) column,the mobile phase was 1% acetic acid water solution-acetonitrile (7∶3) with the flow rate of 0. 8 ml·min-1 . The detection wavelength was 280nm , the column temperature was 30℃ and the injection volume was 20 μl. Results: The linear range of protocatechualdehyde, rosmarinicacid and salvianolic acid A was 3-300 μg·ml-1(r>0. 999 0), and the recovery was within the range of 95. 22%-99. 68% with RSD below 2% (n=5). Conclusion:The method is accurate, rapid and reproducible, and can be used to control the quality of Salvia miltiorrhiza Bunge.
