Quick Detection of Four Isoflavone Compounds from Extracts of Pueraria Lobata Flowers by HPLC
- VernacularTitle:HPLC法快速测定葛花提取物中4种异黄酮类化合物的含量
- Author:
Jinfeng WANG
;
Ying WEI
;
Fang WANG
;
Cuiyan YANG
;
Guoyu WANG
;
Yanping ZHANG
;
Dan LIU
;
Nan ZHAO
;
Yanping WANG
- Publication Type:Journal Article
- Keywords:
Flos puerariae lobata;
Daidzin;
Tectoridin;
Daidzein;
Tectorigenin;
HPLC
- From:
China Pharmacist
2014;(9):1496-1498
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for the determination of four kinds of isoflavone compounds including daid-zin, tectoridin, daidzein and tectorigenin in the extracts of Pueraria lobata flowers. Methods:The separation was performed on an Agi-lent C18 column(250 mm × 4. 6 mm, 5 μm) using a mobile phase of methanol-acetonitrile-water(2∶1∶2). The flow rate was 1. 0 ml· min-1 ,and the detection wavelength was 264nm. The column temperature was 25℃ and the sample size was 20 μl. Results:The de-tection could be accomplished within 10 minutes with good separation and specificity of four isoflavone compounds with the retention timeof3.4,3.8,5.7and7.2min,respectively. Thelinearrangewas0.784-78.440 μg·ml-1(daidzin),2.000-200.000 μg· ml-1(tectoridin) and 0.800-80.020 μg·ml-1 (daidzein and tectorigenin),and the relative coefficient was 0.999 9, 0.999 8, 0. 999 7 and 0. 999 9, respectively. The average recovery was 100. 54%(RSD=1. 66%,n=6),100. 03%(RSD=1. 00%, n=6), 99. 48%(RSD=1. 76%, n=6) and 100. 92%(RSD=2. 26%, n=6), respectively. Conclusion: The method is simple, rapid and accurate with good repeatability, which can be used in the rapid determination of isoflavone compounds in the flowers of Pueraria loba-ta.
