Quantitative Determination of Two Components in Isoniazid and Vitamin B6 Tablets by HPLC
- VernacularTitle:HPLC法测定异烟肼维B6片中两组份的含量
- Author:
Ke HUANG
- Publication Type:Journal Article
- Keywords:
Isoniazid and vitamin B6 tablets;
Isoniazid;
Vitamin B6;
Assay;
HPLC
- From:
China Pharmacist
2014;(9):1510-1512
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a determination method for isoniazid and vitamin B6 tablets by HPLC. Methods: The proce-dure was performed on an Agilent Eclipse Plus C18 (250 mm × 4. 6 mm,5 μm) column at 30℃ and the detection wavelength was 291 nm. The mobile phase was composed of 0. 1% triethylamine [ in which 20 mg sodium 1-octanesulfonate was added and the pH value was adjusted to (3. 0 ± 0. 05) by phosphoric acid] and methanol (70∶30) at a flow rate of 1. 0 ml·min-1 , and the injection volume was 10 μl. Results:The linear range of isoniazid and vitamin B6 was within the range of 2. 535-50. 700 μg(r=1. 000 0)and 0. 137-2. 735 μg(r=1. 000 0), respectively. The mean recovery was 99. 57%(RSD=0. 89%) and 99. 59%(RSD=0. 43%) (n=9), re-spectively. Conclusion:The method is specific, accurate, sensitive and simple, which is applicable in the quality control of isoniazid and vitamin B6 tablets.
