Content Determination of Tetrahydropalmatine and Imperatorin in Yuanhu Zhitong Tablets
- VernacularTitle:元胡止痛片中延胡索乙素和欧前胡素的含量测定
- Author:
Jing YANG
;
Fei MA
;
Haixia LI
;
Feifei REN
- Publication Type:Journal Article
- Keywords:
Yuanhu Zhitong tablets;
Tetrahydropalmatine;
Imperatorin;
HPLC;
Determination
- From:
China Pharmacist
2014;(9):1512-1514
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for the content determination of tetrahydropalmatine and imperatorin in Yuanhu Zhi-tong tablets by HPLC. Methods:An AKZONOBEL Kromasil 100-5 C18 column (250 mm × 4. 6 mm , 5μm) was used and the mobile phase consisted of methanol and 0. 1% phosphoric acid (52∶48) (adjusting pH to 6. 5 with triethylamine),the flow rate was 1. 0 ml· min-1 at the column temperature of 40℃, the detection wavelength was at 284 nm, and the injection volume was 10 μl. Results:The linear range of tetrahydropalmatine was 0.004 2-0.084 0 mg·ml-1(r=1.000 0). The average recovery was 97.72% with RSD of 1.10%(n=6). The linear range of imperatorin was 0.003 6-0.072 0 mg·ml-1(r=0.999 7). The average recovery was 97.99%with RSD of 0. 96%(n=6). The measurement results of 10 batches were stable and reliable. Conclusion:The method is simple and accurate, which can be used to improve the quality standard.
