Determination of Rutin in Anoectochilus Roxburghii by HPLC
- VernacularTitle:HPLC法测定金线莲中芦丁的含量
- Author:
Weifeng ZHENG
;
Zhiyuan LIU
- Publication Type:Journal Article
- Keywords:
Anoectochilus roxburghii;
HPLC;
Rutin;
Content
- From:
China Pharmacist
2014;(9):1584-1585
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for the determination of rutin in Anoectochili roxburghii. Methods:The detection was carried out by HPLC. A Lanbo-Kromasil C18 column(250 mm × 4. 6 mm,5 μm) was used with methanol-0. 2% phosphoric acid solu-tion (40∶60) as the mobile phase;the detection wavelength was 257nm, the flow rate was 1. 0 ml·min-1 and the column temperature was 30℃. Results:Rutin showed a good linear relationship within the range of 0. 021-0. 433 μg(r=0. 999 6). The average recovery was 96. 65%(RSD=1. 67%, n=9). Conclusion:The method is simple, rapid, specific and reproducible.
