Carboxymethytl pachymaram induces the methylation of SOCS-1 gene and the maturation of human monocyte-derived dendritic cells
10.3760/cma.j.issn.0254-5101.2014.08.006
- VernacularTitle:羧甲基茯苓多糖对人外周血源性树突状细胞SOCS-1基因甲基化及体外成熟的影响
- Author:
Gaochao QIAN
;
Wei PAN
;
Xiaojing TIAN
;
Zhixiang DING
;
Wentao JIN
;
Qi ZHANG
- Publication Type:Journal Article
- Keywords:
Carboxymethytl pachymaram;
Dendritic cell;
Suppressor of cytokine signaling-1 (SOCS-1);
Methylation
- From:
Chinese Journal of Microbiology and Immunology
2014;(8):599-603
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of carboxymethytl pachymaram ( CMP ) on the methylation of SOCS-1 (suppressor of cytokine signaling-1) gene and the in vitro maturation of human mono-cyte-derived dendritic cells (DCs).Methods Human DCs were induced from the peripheral blood mono-cytes in vitro with the treatment of recombined human GM-CSF and interleukin-4 ( IL-4 ) and cultured with different concentrations of CMP (10, 50, and 100 mg/L).The methylation and expression of SOCS-1 gene were analyzed by methylation-specific polymerase chain reaction (MSP) and real-time PCR, respectively. The phenotypic markers of DCs were detected by flow cytometry .Mixed lymphocyte reaction ( MLR) and ELISA were performed to measure the lymphocyte proliferation induced by DCs and IL-12 secretion by DCs . Results CMP promoted the methylation of SOCS-1 gene, but inhibited the expression of SOCS-1 gene in dendritic cells at the concentrations of 50 mg/L and 100 mg/L.The expression of phenotypic markers (CD80, CD83, CD86 and HLA-DR), IL-12 secretion and lymphocyte proliferation induced by DCs were significantly enhanced in a dose dependent manner with the treatment of CMP .Compared with control group , the levels of methylated SOCS-1 gene and IL-12 and the lymphocyte proliferation index were increased upon the stimulation with 50 mg/L and 100 mg/L of CMP (P<0.01), but the expression of SOCS-1 gene was de-creased.The expression of CD80, CD83 and HLA-DR on DCs in the presence of 100 mg/L of CMP were higher than those of control group (P<0.05).Conclusion CMP could induce the methylation of SOCS-1 gene and the maturation of DCs derived from peripheral blood monocytes .
