Determination of Menthol in Xiao’ er Fufang Mahuangjian Jiegeng Syrups by HS-GC
- VernacularTitle:顶空气相色谱法测定小儿复方麻黄碱桔梗糖浆中薄荷脑的含量
- Author:
Chengshuai WANG
;
Jinhuang YU
;
Xiaoyan ZHANG
- Publication Type:Journal Article
- Keywords:
HS-GC;
Xiao’ er Fufang Mahuangjian Jiegeng syrups;
Menthol
- From:
China Pharmacist
2014;(9):1592-1594
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HS-GC method for the determination of menthol in Xiao ’ er Fufang Mahuangjian Jiegeng syrups. Methods:An Agilent DB-WAX column(30 m × 0. 53 mm,1. 0 μm)with an FID detector was used;the inlet temperature was 150℃, the detector temperature was 250℃;the flow rate of the carrier gas ( N2 ) was 2. 0 ml·min-1 , the split ratio was 1∶1; the headspace equilibrium temperature was 80℃, the balance time was 30 min, and the sample volume was 1ml. Results:The calibration curve of menthol was within the range of 2. 09-20. 92 μg·ml-1, which showed a good linear relationship (r=0. 999 1). The average recovery of menthol was 98. 0%(RSD=1. 8%, n=6). Conclusion: The method is simple, accurate and reproducible, and can be used for the determination of menthol in Xiao’ er Fufang Mahuangjian Jiegeng syrups.
