Expression and signification of cell cycle regulation protein Cyclin D1-CDK4-p21 in scar cancer
- VernacularTitle:细胞周期调控系统相关因子 Cyclin D1-CDK4-p21在瘢痕癌中的表达及意义
- Author:
Yujing LIN
;
Ruizhen GUO
;
Haiqing WANG
- Publication Type:Journal Article
- Keywords:
Scar cancer;
Cell cycle regulation;
Cyclin D1;
CDK4;
p21
- From:
Journal of Medical Postgraduates
2014;(9):923-927
- CountryChina
- Language:Chinese
-
Abstract:
Objective Dysfunction of cell cycle regulation is one of the key factors for cellular carcinogenesis .This paper aimed to study the expression and significance of cell cycle regulation protein Cyclin D 1-CDK4-p21 in scar cancer . Methods The expressions of Cyclin D1, CDK4 and p21 protains were detected in scar cancer group , pathological scar group and normal skin group respectively by using immunohistochemical staining (SP).The mRNA expression levels of Cyclin D1, CDK4 and p21 were detected by the use of nucleic acid-mediated in-situ hybridization .Correlation analysis was made on the indexes , and the average optical density and positive area were analyzed using image analysis . Results The expressions of Cyclin D1, CDK4 and p21 protains and the mRNA ex-pression levels of cyclin D1, CDK4 and p21 were high in scar cancer group, low in pathological scar group , and negative in normal skin group.The mean optical density and positive area in scar cancer group were significantly different from pathological scar group and normal skin group (P<0.01).But no significant difference was found be-tween pathological scar group and normal skin group (P>0.05).In terms of correlation analysis , the expressions of Cyclin D 1 and CDK4 as well as p21 and CDK4 in scar cancer tissue were both in posi-tive correlations. Conclusion The occurrence of scar cancer is related to the abnormal expression of Cyclin D 1 and CDK4.The complex formed by Cyclin D1 and CDK4 may promote the G1/S transition, proliferation and tumorigenesis of scar cancer .In scar canc-er, the inhibition of Cyclin D1-CDK4 complex might be caused by other members of CKI family or even inbibitors of other families apart from CDK family.
