Screening protein interacted with enterovirus 71 3A protein by means of T7-phage display system
10.3969/j.issn.1673-4130.2014.16.004
- VernacularTitle:噬菌体展示技术筛选人肠道病毒71型3A 蛋白相互作用蛋白
- Author:
Rui TANG
;
Yongbo YANG
- Publication Type:Journal Article
- Keywords:
bacteriophage T7;
enterovirus 71;
3A protein
- From:
International Journal of Laboratory Medicine
2014;(16):2129-2131
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen and identify protein interacted with enterovirus 71 (EV71)3A protein by means of T7-phage display system.Methods The prokaryotic expression vector of 3A protein was constructed for expressing and purifying 3A pro-tein.With 3A protein as the target protein,the T7-phage display system was adopted to screen the human hepatocyte cDNA library. The screened products were performed the DNA sequence analysis and the homologous research.Results 3A protein was expressed and purified,after 4 rounds of biopanning,37 positive clones were selected.The inserted fragments were amplified by PCR using specific T7 primer.The products were sequenced,2 proteins were identified to interact with 3A protein by homologous analysis. Conclusion Trough the T7 phage display system,two proteins interacted with 3A are obtained.The possible function of 3A protein can be speculated by researching the function of these two proteins,which lays the foundation for the further studying the pathogen-ic mechanism of EV71.
