Quantitative analysis on EB virus DNA in peripheral white blood cell,plasma and serum in patients with hematologic diseases
10.3969/j.issn.1673-4130.2014.16.017
- VernacularTitle:血液病患者外周血白细胞、血浆和血清中 EB 病毒 DNA 定量分析
- Author:
Kedi WANG
;
Zhi LV
;
Tieshan WANG
;
Jianrong SU
- Publication Type:Journal Article
- Keywords:
EB virus;
peripheral white blood cell;
serum
- From:
International Journal of Laboratory Medicine
2014;(16):2161-2162
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the load of EB virus in peripheral white blood cell,plasma and serum in the patients with he-matologic diseases,and to discuss the feasibility of detecting EB virus load by using plasma or serum instead of peripheral white blood cell.Methods Venous blood of 125 patients with hematologic diseases were collected,and peripheral white blood cell,plasma and serum were isolated.The real-time quantitative PCR was used to detect the virus load in three kinds samples with the EB virus load in peripheral blood cell as the gold standard.Results Compared to peripheral white blood,the EB virus load in plasma and ser-um showed no statistical difference(P >0.05).Conclusion Using plasma or serum instead of peripheral white blood cell for con-ducting the quantitative detection of the load of EB virus will be a reliable method.
