Comparison of qualitative and quantitative detections of urine NGAL in patients with diabetic nephropathy
10.3969/j.issn.1673-4130.2014.16.018
- VernacularTitle:糖尿病肾病患者尿液 NGAL 蛋白的定性及定量检测比较
- Author:
Xiaojie HE
;
Shenren CHEN
;
Yihua YANG
;
Donghai GU
- Publication Type:Journal Article
- Keywords:
neutrophil gelatinase-associated lipocalin;
diabetic nephropathy;
urinary albumin excreting rate
- From:
International Journal of Laboratory Medicine
2014;(16):2163-2165
- CountryChina
- Language:Chinese
-
Abstract:
Objective To ascertain the significance of qualitative and quantitative detections of urine neutrophil gelatinase-associ-ated lipocalin(NGAL)in the early diagnosis of diabetic nephropathy.Methods 74 patients with diabetes mellitus were divided into the normoalbuminuria group[(DN1 ,n =26,urinary albumin excretion rate(UAER)<30 mg/24 h],the microalbuminuria group (DN2 ,n=24,UAER 30-300 mg/24 h)and the macroalbuminuria group(DN3 ,n=24,UAER>300 mg/24 h)according to the 24 h UAER,and at the same time the control group(n=25)was set up.Both the activity and levels of urine NGAL in the above groups were examined and compared.Results The activity band of urine NGAL in each group were more distinct than that of the control group(P =0.000).The NGAL activity in the macroalbuminuria group was higher than that in the normoalbuminuria group(P <0.05).No difference in the NGAL activity was found between the micro-and normal-albuminuria groups(P >0.05);the quantitative detection demonstrated that differences of the NGAL content existing between the macroalbuminuria group and the other three groups had statistical significance(P =0.000),the urinary NGAL excretion in the macroalbuminuria group was higher than that in the microalbuminuria group,normoalbuminuria group and the control group(P =0.000);No differences were found between the control group and the normoalbuminuria group and between the control group and the microalbuminuria group(P >0.05).Conclu-sion For the patients with diabetic nephropathy,the activity detection of urine NGAL is more sensitive than UAER and the NGAL quantitative detection and could become a new type index in the early diagnosis of diabetic nephropathy.
