Clinical analysis of real-time fluorescent PCR technique and bacterial culture for detecting colonization of group B Streptococcus in late pregnant women
10.3969/j.issn.1673-4130.2014.16.043
- VernacularTitle:实时荧光 PCR 技术和细菌培养法检测妊娠晚期孕妇定植B 群链球菌临床分析
- Author:
Li WANG
;
Wei YE
;
Jie MA
- Publication Type:Journal Article
- Keywords:
group B streptococcus;
polymerase chain reaction;
germiculture
- From:
International Journal of Laboratory Medicine
2014;(16):2220-2221
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the sensitivity of the real-time fluorescence PCR technique and the bacterial culture for detecting the colonization of group B Streptococcus(GBS)in late pregnant women.Methods 2 specimens were collected from preg-nant women genital tract-rectal secretions swabs,one specimen for conducting the bacterial culture and another for conducting the real-time PCR technique to detect genital GBS.The accuracy and rapidness were compared between the two methods.308 cases of pregnant women were divided into the GBS positive group and the GBS negative group according to the detection results of the real time real-time fluorescence PCR technique.The relation between the occurrence of premature rupture of membranes with GBS was investigated by the comparative analysis.Results Among 308 pregnant women with GBS detection,18 cases were positive by the ordinary bacterial culture with the positive rate of 5.8%(18/308),while 28 cases were positive by the real-time fluorescent PCR with the positive rate of 9.4%(29/308).In the GBS positive group detected by PCR,the premature rupture of membranes occurred in 9 cases with the positive rate of 31%,while in the GBS negative group detected by PCR,which occurred in 33 cases with the pos-itive rate of 11.83%.Conclusion This survey shows that the positive detection rate of the real-time fluorescent PCR technique is significantly higher than that of the bacterial culture method,the application of this detection technique for detecting GBS provides the basis for rapidly diagnosing GBS and conducting the prophylactic use of antibacterial drugs more accurately and more effectively.
