Comparison of the Effects of Buyang Huanwu Decoction and Its Recipe Composition on Cerebral Ischemic Injury
10.3969/j.issn.1005-5304.2014.09.014
- VernacularTitle:补阳还五汤及其精简方抗脑缺血损伤功效比较
- Author:
Fang LIU
;
Qingping LIU
;
Yuhong WANG
;
Guangxian CAI
;
Xiangyi XIA
;
Le SHAO
;
Shaolong LIU
- Publication Type:Journal Article
- Keywords:
Buyang Huanwu Decoction;
recipe composition of Buyang Huanwu Decoction;
cerebral ischemic injury;
MCAO rat model;
oxidative stress cell model
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(9):46-49
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the preventive effects of Buyang Huanwu Decoction (BYHWD) and its recipe composition (BYJJF) in focal ischemic brain injury condition in vivo/in vitro. Methods In vivo studies, SD rats were divided into sham-operation group, MCAO group, BYHWD group and BYJJF group based on rat weight, 10 rats in each group. The body weight, infarct area and brain water contents were determined. In vitro studies, H2O2 was used to damage PC12 cells, and the vitro oxidative stress cell model was established. PC12 cells were divided into normal group, blank control group, BYHWD and BYJJF groups with different concentrations (0.1, 0.2, 0.5, 1.0, 2.0, 3.5 mg/mL). MTT method was employed to determine the protective effects of BYHWD and BYJJF on model cells.Results Vivo studies showed that after 7 days of treatment with BYHWD and BYJJF, those determinated quotas were all significantly improved compared with MCAO model rats (P<0.05), and there were no differences between BYHWD and BYJJF (P>0.05).Vtiro studies showed that the protective effects of BYHWD and BYJJF took place 2 hours later, and it was obvious in oxidative stress injury caused by H2O2, with statistical differences with model group (P<0.05). BYHWD and BYJJF could increase cell viability, and there was no difference between the groups with same concentration (P>0.05).Conclusion The research confirmed that BYJJF plays a significant role in improving the cerebral ischemia injury, which is the same performance as BYHWD, and BYJJF can save TCM resources under the precondition of TCM efficacy.
