Effect of low-level 84 disinfection solution on reproductive physiology of mice
10.3969.j.issn.1671.7856.2014.007.008
- VernacularTitle:低浓度84消毒液对小鼠生殖生理的影响
- Author:
Yu HU
;
Yun TIAN
;
Li ZHOU
;
Jun ZHOU
;
Zhigang DING
- Publication Type:Journal Article
- Keywords:
84 Disinfection solution;
Mice;
Reproductive physiology
- From:
Chinese Journal of Comparative Medicine
2014;(7):41-44
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of low-level 84 disinfection solution on reproductive physiology of mice . Methods 72 KM mice were randomly divided into three groups :0.5%84 disinfection solution group ( group A) , 2.0%84 disinfection solution group (group B) and saline solution group (group C).12 males and 12 females in each group. Corresponding medicine was given once a day by intragastric gavage for 9 weeks.The estrous cycle of female mice was observed by exfoliative cytology of vagina .After 9 weeks, The sperm abnormality test was performed , Serum samples were taken for sex hormone test by ELISA, and the histological change of testis (or ovary), heart, liver, lung and kidney were observed.Results Compared with group C , No significant difference was observed in the weight variation and serum sex hormone level in group A and B (P >0.05).The difference of the estrous cycle of female mice was not significant (P >0.05).No histopathological abnormality was observed .Compared with group C, There was no significant difference in the rate of sperm abnormality in group A ( P >0.05 ) , but the rate in group B was significantly increased ( P <0.05 ) . Conclusion 0.5% 84 disinfection solution has no significant effect on reproductive physiology of mice , The concentration of disinfection solution must be strictly controlled when used .
