Effects of salvianolic acid A injection on CCl4 induced hepatocyte injury and hepatic fibrosis in rats
10.3969.j.issn.1671.7856.2014.007.012
- VernacularTitle:注射用丹酚酸A防治肝纤维化的实验研究
- Author:
Ting LU
;
Fangming CHEN
;
Keyan ZHU
;
Yueqin CAI
;
Qiyang SHOU
;
Yongming PAN
;
Minli CHEN
- Publication Type:Journal Article
- Keywords:
Salvianolic acid A;
Hepatic fibrosis;
Anti-lipid peroxidation;
CCl4
- From:
Chinese Journal of Comparative Medicine
2014;(7):57-62
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study on the anti-hepatic fibrosis effects of salvianolic acid A injection (SAA), and further to provide the theoretical basis for the clinical application .Methods Using CCl4 induced hepatocyte injury in vitro, the hepatocyte viability , the levels of ALT , AST and LDH in cell culture supernatants and the levels of SOD and MDA in cell lysates were detected .In addition , the hepatic fibrosis rat model was made by subcutaneous injection of CCl 4 , the serum LN, HA, SOD and MDA levels were detected and the pathological changes in liver tissue were also observed . Results Compared with model group , the hepatocyte viability in SAA high or low dose group and Vit E group were significantly increased (P <0.01), and the activities of ALT, AST and LDH in SAA high dose group were significantly lowed ( P <0.01 ) .The activity of SOD in SAA high dose group and Vit E group was significantly increased ( P <0.05), while MDA content was decreased (P <0.05).Vivo test showed that the levels of serum LN and HA in SAAhigh dose group were significant lower than those of hepatic fibrosis rat model group (P <0.05).Moreover, the activity of SOD in SAA high or low dose group was significantly increased (P <0.05, P <0.01), while MDA content was lowed (P <0.05, P <0.01), and can improve the pathological of liver tissues .Conclusions SAA injection can anti-lipid peroxidation and thereby protect hepatocyte and reduce hepatic fibrosis .
