The clinical value of detection of fluorescence quantitative PCR in detection of HBV DNA and HBV markers
10.3969/j.issn.1673-4130.2014.16.041
- VernacularTitle:荧光定量 PCR 法检测乙肝病毒 DNA 和乙肝病毒标志物检测的临床价值分析
- Author:
Haiyan CHEN
;
Guihua ZHANG
;
Jinbin LUO
;
Shuting HUANG
- Publication Type:Journal Article
- Keywords:
enzyme linked immunosorbent assay;
polymerase chain reaction;
hepatitis B virus
- From:
International Journal of Laboratory Medicine
2014;(16):2216-2217
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the clinical value of detection of fluorescence quantitative PCR in detection of HBV DNA and HBV markers.Methods 653 hepatitis B patients in our hospital from 2013 April to 2013 December were selected.First ELISA method using HBV-M model for qualitative detection of the blood samples,the detection order:Hepatitis b virus surface antigen (HBsAg),Hepatitis B surface Antibody(HbsAb),Hepatitis B e Antigen(HbeAg),hepatitis Be antibody(HbeAb),hepatitis B core antibody(HbcAb);Then the FQ-PCR method for the quantitative detection of HBV-DNA,different HBV-m model test results were compared..Results Big 3 this world[1(+)、3 (+)、5 (+)model]and[1 (+)、3 (+)model ]of the HBV-DNA positive rate was 97.97%,94.74% was significantly higher than that in other mode(P<0.05).Big 3 this world[1(+)、3(+)、5(+)model]HBV-DNA expression level is the highest(5.59 ×106 ±2.42 ×105 )copies/mL was significantly higher than that in other mode(P<0.05).Conclusion Combined with qualitative and quantitative detection of HBV-DNA HBV-M,which is of great value to clinical early diagnosis and therapy.
